Figure 3
Characterization of tandem c-di-GMP-I using dual-fluorescence reporter in E. coli BL21(DE3).
(a) Schematic diagrams of amcyan-Bc3-5-turborfp sandwich-like dual-fluorescence reporter. IPTG induced expression of PleD (orchid ellipsoid dimer). Accumulating c-di-GMP trigger the c-di-GMP responsive riboswitch to turn on TurboRFP (red ellipsoid dimer) expression, giving rise to TurboRFP fluorescence versus the constitutive AmCyan fluoresence (bright green ellipsoid tetramer). (b) Read-through rate comparisons among single, double- and triple-tandem c-di-GMP-I. Strains were listed in Table 1. All data were collected from three independent experiments (error bars were standard deviation from mean values). (c) The theoretical read-through rate curves of single, double- and triple-tandem c-di-GMP-I. (d) The theoretical read-through rate equations of single, double- and triple-tandem c-di-GMP-I. TD, the equilibrium constant equal to the c-di-GMP concentration (C(c-di-GMP)) at 50% read-through rate.
