Figure 4 | Scientific Reports

Figure 4

From: Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter

Figure 4

Correlation between intracellular c-di-GMP concentration and RFI.

(a) Quantification of c-di-GMP concentration of Bc3-5/pleD strain under IPTG induction at different levels. (b) RFI values of Bc3-5/pleD and Bc3-5/pET strains under IPTG induction at different levels. (c) Correlation between intracellular c-di-GMP concentration and RFI. (d) Visible fluorescence of Bc3-5/pleD and Bc3-5/pET concentrated bacterial suspensions under a bright field. Strains were listed in Table 1. Bacterial culture, concentration, resuspension and photography were carried out as described in the Methods section. RFI was calculated as the ratio of fluorescence intensity at 489 nm to fluorescence intensity at 547 nm. All Data were averages of three independent experiments (error bars were standard deviation from mean values).

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