Figure 3: Enzymatic activity and oligomerization state of PfTET3 is modulated by M1.
From: Tuned by metals: the TET peptidase activity is controlled by 3 metal binding sites
(A) EDTA titration of PfTET3 activity towards 5 mM Lys-pNa at T = 85 °C. Data are fitted by a bi-dose responsive curve from Origin 8.5 (red line). Error bars are standard deviations of three independent measurements. (B) Gel filtration (GF) profiles of PfTET3Zn heated at 85 °C in the absence of EDTA (black line, dodecamer), with 15 μM EDTA (red line, 60% dodecamer and 40% monomer from GF profile)) and with 6 mM EDTA (blue line, monomer). (C) Co2+ titration of PfTET3 activity towards 5 mM Lys-pNa at T = 85 °C (black squares), T = 50 °C (red squares) and T = 20 °C (blue squares). Co2+ enhances the PfTET3 activity only at 85 °C.
