Figure 5: Temperature dependence of PfTET3 activity.
From: Tuned by metals: the TET peptidase activity is controlled by 3 metal binding sites
(A) Arrhenius plot of PfTET3Zn between 20 °C–85 °C. The breakpoint is at 35 °C, suggesting a reduced flexibility of PfTET3Zn in the active site and at the interfaces. The non-linear profile indicates cold inactivation by rigidifying the oligomeric interface. (B) PfTET3 active site. Blue and red spheres stay for Co2+ and Zn2+, respectively. For the sake of the clarity, only residues involved in salt bridges with Glu207 are labeled. The dashed lines are the electrostatic interactions between Glu207 and Gly288/Asp230. Such interactions increase the pKa of Glu207 and decrease the enzymatic activity of PfTET3 at 20 °C.
