Figure 6: The M3 site broaden the PfTET3 substrate preferences.
From: Tuned by metals: the TET peptidase activity is controlled by 3 metal binding sites
(A) P1 pocket of PfTET3 in electrostatic surface representation. Blue spheres are Co2+ ions, red sphere is a Zn2+ ion. For the sake of the clarity, only the residues interacting with the M3 site are highlighted. The presence of M3 modify the electrostatic properties of P1 allowing the degradation of glutamate. (B) Substrate specificity of PfTET3 is expanded towards glutamate in the presence of Co2+ (blue rectangles) at 85 °C. In the absence of Co2+ (red rectangles) PfTET3 is almost inactive. Error bars are standard deviations of three independent measurements.
