Table 4 Temperature dependence of PfTET3 kinetic parameters.

From: Tuned by metals: the TET peptidase activity is controlled by 3 metal binding sites

 

Km (mM)

kcat (s−1)

kcat/Km (s−1 M−1)

PfTET3Co 85 °C

0.62 ± 0.07

2436 ± 191

3.9*106

PfTET3Zn 85 °C

7.0 ± 1.41

2890 ± 412

4.1*105

PfTET3Zn 67.5 °C

8.7 ± 0.6

3500 ± 183

4.0*105

PfTET3Zn 50 °C

4.0 ± 0.6

959 ± 101

2.4*105

PfTET3Zn 35 °C

5.0 ± 0.8

707 ± 81

1.4*105

PfTET3Zn 20 °C

4.0 ± 0.9

45.7 ± 0.37

1.1*104