Figure 2: Colon cancer cells HCT116 showed lower proliferative and migratory capacity when treated with 5-Fu/P85 copolymer micelle. | Scientific Reports

Figure 2: Colon cancer cells HCT116 showed lower proliferative and migratory capacity when treated with 5-Fu/P85 copolymer micelle.

From: Inhibition of Growth and Metastasis of Colon Cancer by Delivering 5-Fluorouracil-loaded Pluronic P85 Copolymer Micelles

Figure 2

(A) The cell viability of HCT116 cells treated by different drug groups was measured by cell counting Kit-8(CCK-8). The OD values were detected by microplate reader at 450 nm. (B) Clonogenic assay was performed to investigate the clone forming ability of HCT116 cells treated with 5-Fu/P85 copolymer micelle. The cells in six well culture plates were stained by crystal violet. (C,D) Cell migration ability was tested by wound-healing assay and transwell migration assay. (E) Transwell invasion assay was performed to detect the invasive ability of HCT116 cells. The boyden chambers were stained by crystal violet in transwell migration and invasion assays. The number of cells was counted under a microscope. (Images in (C–E) were taken by microscope under ×200 magnification. The concentration of 5-Fu was 0.025 mg/ml for 5-Fu/P85 copolymer micelle. *P < 0.05; **P < 0.01.).

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