Figure 5: Administration of 5-Fu/P85 copolymer micelles significantly decreased the content of CD133 + CXCR4+ cells in HCT116 cancer cells and inhibited the colony-forming ability of CD133 + CXCR4+ cells.

(A)The percentage of CD133 + CXCR4+ cells was analyzed by flow cytometry. (B) The clonogenic capacity of CD133 + CXCR4+ cells was investigated by CFU assay. The cells in six-well culture plate were stained by crystal violet and the number of colonies containing ≥20 cells was counted under a microscope. (C) Quantitative bar graphs showing CD133 + CXCR4+ cell counts determined by flow cytometry. (**P < 0.01;***P < 0.001).