Figure 1: Characterization of syntabulin-PICK1 interaction.
From: Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons
(A) Diagram of syntabulin and PICK1 proteins. The amino acid numbers are indicated above the bar diagram, and the coverage of partial domains is indicated below. Stb, syntabulin; FL, full-length; KBD, kinesin-binding domain; SBD, syntaxin-binding domain; MBD, mitochondrion-binding domain; Nt, N-terminus; Ct, C-terminus. (B) Yeast-two-hybrid result of syntabulin-PICK1 interaction domain mapping: −, no clear yeast colony growing on SCM-3 plates; ++, positive colonies growing on SCM-3 plates; +++, positive colonies growing on SCM-4 plates. The result shows an interaction between syntabulin SBD and PICK1 BAR. (C) Coimmunoprecipitation of syntabulin domain fragments and PICK1 from lysates of HEK293T cells overexpressing these proteins. The results show a strong interaction between syntabulin SBD and PICK1. (D) Coimmunoprecipitation of PICK1 domain fragments and syntabulin overexpressed in HEK293T cells. The results show that PICK1 BAR domain exhibits a strong binding.
