Figure 2: Syntabulin recruits PICK1 onto microtubule structures.
From: Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons
(A) Expression pattern of myc-tagged syntabulin full-length or partial fragments in COS7 cells. (B) Myc-Syntabulin-FL or the N-terminal fragment containing both KBD and SBD can induce microtubule bundles. β-tub, β-tubulin. (C) COS7 cells were transfected with GFP-PICK1 together with or without myc-Syntabulin-FL/partial-fragment constructs, and then stained with myc and β-tubulin antibodies. The results show that Syntabulin-FL and -Nt fragments were able to recruit PICK1 onto microtubule structures. The SBD of syntabulin formed coclusters (arrowheads) with PICK1 and showed no clear relationship with microtubules. Scale bar = 10 μm in all panels.
