Figure 5: Syntabulin mediates dynamic movement of PICK1 along axons.
From: Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons
(A) Time-lapse imaging of GFP-PICK1 expressed alone or together with syntabulin in neurons. Left panels show the original PICK1 distribution in axons. Right panels show the kymograph of PICK1. (B) Classification of PICK1 vesicles in axons. The result shows that the population of mobile PICK1 vesicles increased upon syntabulin coexpression; n = 4 independent experiments. (C) Cumulative probability of PICK1 distance moved in 5 min of time-lapse imaging; >1000 vesicles from 4 independent experiments were quantified. (D) Neurons were infected with lentivirons knocking down endogenous syntabulin and expressing YFP-PICK1 simutaneously. Time lapse imaging of YFP-PICK1 were carried out to examine the PICK1-containing vesicle dynamics. Scale bars are as indicated. (E) Quantification of the population of PICK1-containing vesicles. Result showed significant reduction of mobile PICK1 vesicles after syntabulin knockdown. >700 vesicles from 3 independent experiments were quantified for each group.
