Figure 7: Syntabulin and PICK1 selectively form complex with ASIC2a in vitro.
From: Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons
(A) GFP-Syntabulin, myc-ASIC2a, or myc-ASIC1a was transfected into HEK293T cells together with or without myc-PICK1, and immunoprecipitated with GFP antibody. Syntabulin showed higher binding affinity for the ASIC2a-PICK1 complex than for the ASIC1a-PICK1 complex. (B) COS7 cells were transfected with PICK1, ASIC1a/2a, and syntabulin in different combinations (as indicated). mch: mcherry. PICK1 and ASIC1a/2a formed numerous coclusters in the cytosol when coexpressed in COS7 cells (top panels). Syntabulin showed no clear colocalization with ASIC1a/2a (middle panels). Syntabulin, PICK1 and ASIC2a but not ASIC1a showed a microtubule distribution pattern when expressed together (bottom panels). Scale bar = 10 μm.
