Figure 2
From: cJun N-terminal kinase (JNK) phosphorylation of serine 36 is critical for p66Shc activation
JNK1/2 deficiency results in an impaired p66ShcS36 phosphorylation upon HR and prooxidant treatment.
JNK1/2+/+ and JNK1/2−/− MEFs were treated with H2O2 (1 mM, 30 min) (a) or HR (hypoxia 90 min, reoxygenation 10 min) (b). Cell lysates obtained from the same experiment and run on a single gel were subjected to the analysis for p66Shc S36 phosphorylation by immunoblotting and representative blots are shown. Summary graphs of results obtained in at least three independent experiments are provided. The band intensity observed in control samples was arbitrarily set to 1. Original blots have been cropped and full length blots are shown in the Supplementary Figure S4. Statistical analysis was performed using t-test (**p < 0.01, ***p < 0.001).
