Figure 5
From: cJun N-terminal kinase (JNK) phosphorylation of serine 36 is critical for p66Shc activation
PKC is not the upstream kinase of JNK.
JNK+/+ MEFs were treated either with Gö6976 (1 μM) or SP600125 (20 μM) both under HR and H2O2 treatment. Phosphorylation of JNK or its substrates c-Jun and ATF2 was detected by Western blotting (a). Pattern of phosphorylation of JNK under H2O2 and HR was confirmed in PKCß+/+ or PKCß−/− MEFs (b). Representative blots and summary graphs as mean ± SD for at least three individual experiments are shown (*p < 0.05, **p < 0.01, ***p < 0.001).
