Figure 7
From: cJun N-terminal kinase (JNK) phosphorylation of serine 36 is critical for p66Shc activation
JNK1/2 deficiency results increased resistance to cell death induced by HR.
JNK1/2+/+ and JNK1/2−/− MEFs were subjected to HR (hypoxia 3 h, reoxygenation 16 h). Morphological changes were documented under the phase-contrast microscope (a, 100× magnification) and cell death was assessed by trypan blue (d) or Annexin V/PI staining followed by FACS analysis (c). Immunoblotting was performed to analyze the phosphorylation of histone γH2AX and PARP/Casp-3 cleavage (b) (n ≥ 4). Size bar = 100 μM. Statistical analysis was performed using ANOVA (***p < 0.001).
