Figure 1: Dosing system setup and mosquito targeting.

(a) The current dosing laser (here, Coherent Verdi V10) was axially co-aligned with an imaging camera (Blackmagic Cinema Camera EF) using a dichroic beamsplitter that reflected the laser light down to the test subject stand and passed other visible light (provided by LED strips on bottom of optical platform) from the subject stand up through a telescopic macro lens (Canon EF 180mm f/3.5 Macro) and into the camera. Live images from the camera were displayed on a monitor above the setup. The two optical paths projected through an opening in the optical platform onto the (b) floor of a test cage, where anesthetized mosquitoes were arranged in a 12 by 7 grid. The distance from the laser to the subject was approximately two meters. (c) The view from the camera with a crosshair noting the location and extent of the co-aligned laser was used to accurately target the mid-thorax of one subject at a time. (d) Circles indicate which areas of the mosquito were hit by the two typical beam diameters. (e,f) Images from CCD beam profiler of 2.3 mm diameter, 532 nm wavelength spot (e) without and (f) with a subject present validated targeting accuracy and showed that the most intense portions of the Gaussian beam impacted the mosquito. Note that the interference fringes in (e) are from the presence of a glass slide near the sensor to facilitate mosquito placement.