Figure 4 | Scientific Reports

Figure 4

From: Enhancer RNA-driven looping enhances the transcription of the long noncoding RNA DHRS4-AS1, a controller of the DHRS4 gene cluster

Figure 4

RNA pol II and p300 mediate spatial interactions between the DHRS4-AS1 promoter and its enhancer.

(A,B) HepG2 and HL7702 cells were subjected to ChIP to detect changes in enrichment of RNA pol II and p300 at the AS1 enhancer and the DHRS4-AS1 promoter after siAS1eRNA treatment. (C–E) ChIP analysis was performed to investigate the changes in levels of histone modifications H3K27ac and H3K4me1 and transcription factor CEBPbeta in HepG2 cells. Data are shown as fold-enrichment relative to the siRNA-control. P values were determined by Student’s unpaired two-tailed t test. Error bars represent the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. (F) A working model shows the possible role of AS1eRNA on DHRS4-AS1. The enrichment of RNA pol II, p300, H3K4me1, H3K27ac and CEBPbeta establishes open chromatin to produce AS1eRNA. In cooperation with RNA pol II and p300, the AS1 enhancer spatially interacts with the DHRS4-AS1 promoter in an eRNA-dependent manner. The interactions enhance the transcription of DHRS4-AS1. After knockdown of AS1eRNA, the distributions of RNA pol II and p300 at the AS1 enhancer and DHRS4-AS1 promoter are reduced and result in a reduction of DHRS4-AS1 levels.

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