Figure 3 | Scientific Reports

Figure 3

From: Illuminating necrosis: From mechanistic exploration to preclinical application using fluorescence molecular imaging with indocyanine green

Figure 3

The in house-modified fluorescence microscope system and ex vivo verification.

(a) A conventional microscope (Leica M205 FA, LEICA, Germany) was coupled with a low-temperature NIR CCD (PIXIS CCD, Princeton Instruments) and a color camera. A laser generator (MW-GX-785/2W, Changchun FS-Optics technology Co., Ltd., China) was coupled with an optical filtering module embedded in the microscope through an optical fiber, so that the excitation light with a selectable wavelength could be transmitted to an imaging target. The emission was also filtered by the filtering module and then captured by the NIR CCD. The light source provided white-light illumination for the color camera to acquire white-light images. The system can provide 0.5–160× magnification. (b) On the ninth day after ICG injection, the fluorescent image shows two ICG-accumulated areas in the resected mouse hind limb. After TTC staining of the entire limb, the ischemia-reperfusion-caused necrotic tissue locations were visible (white areas), which are the same sites where the ICG accumulated. Scale bar, 5 mm.

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