Figure 3

Homeostatic Synaptic Scaling is impaired in TFR KO Neurons.

(A,B) Surface GluR1 (A) or GluR2 (B) in WT and KO 14 div cultured neurons treated with TTX (1 μM) or bicuculline (20 μM) for 48 hr. Normal growth medium was used as the negative control. Surface AMPA receptor expression is shown using a glow scale from black (zero) to red (low pixel intensity) and white (high pixel intensity). Low-magnification image scale bar = 20 μm. High-magnification image scale bar = 5 μm. Quantification analysis of surface levels of GluR1 (C) or GluR2 (D) in WT and KO neurons in (A,B) after TTX or bicuculline treatment (mean ± SEM). N = 10 cells for each group, *p < 0.05.