Figure 5

Live imaging of NMDA-Induced AMPA Receptor Internalization in TFR KO Neurons.

(A) Time- lapse images of hippocampal neurons transfected with SEP-GluR1 or SEP-GluR2 and subjected to a NMDA (20 μM, 5 min)/washout cycle. Scale bar = 20 μm. Quantification of SEP-GluR1 (B) or SEP-GluR2 (C) fluorescence change in response to NMDA perfusion in (A) (mean ± SEM, n = 6 neurons for each experimental group from three individual experiments). Maximum amplitudes of SEP-GluR1 (D) or SEP-GluR2 (E) fluorescence intensity changes to NMDA stimulation and average recycling half-time (D,E) (T1/2, the time taken from maximum endocytosis to 50% recycling) after NMDA washout (mean ± SEM, n = 6 neurons from three experiments, *p < 0.05, compared with WT neurons, Student’s t test).