Figure 2: Clonogenicity and adipogenicity of the candidate surface markers of mesenteric and subcutaneous in vitro adipogenic cells.

SVF cells from mesenteric WAT (a–e) or subcutaneous WAT (f) were sorted, seeded on 96 well plates at the desired concentration, cultured. After reaching 100% confluency, the cells were treated with adipogenic differentiation medium. The medium was replaced with DMEM containing 10% FBS 2 days after commencement of differentiation; after 48 h, Oil Red O staining was performed and colony- and adipocyte-positive wells were counted. The fraction of Lin⁻ CD29⁺ CD34⁺ cells is shown in the dot plot pictures. The gating hierarchy of Lin⁻ CD29⁺ CD34⁺ cells is shown in Fig. 1. The raw data are shown in Supplementary Dataset S2. The values are expressed as the means ± SD. **p < 0.01; ***p < 0.001. n = 3 in each group.