Figure 3: CFU-F-matched experiment for validating the candidate surface markers of mesenteric and subcutaneous in vitro adipogenic cells. | Scientific Reports

Figure 3: CFU-F-matched experiment for validating the candidate surface markers of mesenteric and subcutaneous in vitro adipogenic cells.

From: Identification of Mouse Mesenteric and Subcutaneous in vitro Adipogenic Cells

Figure 3

SVF cells from mesenteric WAT (a–e) or subcutaneous WAT (f) were sorted, seeded at the same CFU-Fs, cultured and, after reaching 100% confluency, treated with adipogenic differentiation medium. The medium was replaced with DMEM containing 10% FBS 2 days after commencement of differentiation; after 48 h, the medium was collected and Oil Red O staining was performed. The adiponectin concentrations in the medium were assessed. Scale bars, 100 μm. The values are expressed as the means ± SD. *p < 0.05; ***p < 0.001. n = 4–5 in each group.

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