Figure 5: Identification of specific surface markers of mesenteric in vitro adipogenic cells.

Dot plot images of CD9⁻ CD201⁺ Sca-1⁻ cells are shown in (a). CD9⁻ CD201⁺ Sca-1⁻ cells from SVF cells of mesenteric WAT were subjected to in vitro assays ((b) clonogenicity and adipogenicity assays, see Fig. 2 and raw data in Supplementary Dataset S2; (c) Oil Red O staining and adiponectin secretion under CFU-F-matched conditions, see Fig. 3) and in vivo assay (d, transplantation experiments, see Fig. 4; DAPI staining was shown in Supplementary Fig. S3). The term “Control Fraction” indicates CD29⁺ CD34⁺ cells, excluding CD9⁻ CD201⁺ Sca-1⁻ cells. Scale bars, 100 μm. The values are expressed as the means ± SD. **p < 0.01, ***p < 0.001, n = 3 (b,c), n = 5 and 3 (d) in each group.