Figure 4 | Scientific Reports

Figure 4

From: Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device

Figure 4

Left: Flow-cytometry analysis of dissociated HepG2 tumor spheroids with forward-scattering light (FSC), side-scattering light (SSC) and gated areas.

Right: Viability assay using Calcein-AM and 7-AAD (right panel) on dissociated HepG2 tumor spheroids after 3-day culture in the microfluidic devices with cell culture chamber dimensions (width × length) of (A) 200 × 200 μm2 and (B) 300 × 300 μm2 and the height is 250 μm.

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