Figure 5

Bright field microscopic images of the HepG2 tumor spheroids cultured in the microfluidic devices with cell culture chamber dimensions (width × length) of (A) 200 × 200 μm2 and (B) 300 × 300 μm2 and the height is 250 μm for total 3 days and treated with drugs for 48 hours. (C) Quantitative characterization of the diameters of the HepG2 tumor spheroids before and after the drug treatments by imaging analysis on the bright field microscopic images.