Figure 8

Dendritic branching of neurons in pure neuronal culture under treatment with vehicle or different dosages of Na-PA.

(A–E) MAP2 staining of DIV 12 neurons in pure neuronal culture treated with vehicle (A1), 0.3 μM (A2), 1 μM (A3), 3 μM (A4) or 10 μM Na-PA (A5). Bar, 100 μm. (B–E) Quantification of primary dendrites (B), secondary dendrites (C), dendritic tips (D) and Sholl analysis (E) for five groups of neurons in panel A1 to 5, n = 84 for neurons treated with vehicle; n = 73, 78, 82, 68 for neurons treated with 0.3, 1, 3 and 10 μM Na-PA, B-D, *p < 0.05, **p < 0.01, ***p < 0.001, E, F (4, 1552) = 62.54, p < 0.001.