Figure 5: Changes in the profiles of salmon gonadotropin-releasing hormone (sGnRH) and the N-methyl-D-aspartate receptor essential subunit NR1 in the olfactory bulb and telencephalon of adult chum salmon during homing migration.
From: Involvement of hormones in olfactory imprinting and homing in chum salmon
Adult fish were sampled in the Bering Sea (Site G), Ishikari Bay (Site F), the Indian Waterwheel in the Chitose River (Site H), and the Chitose Hatchery (Site A). The gene expression ratios of sGnRH-I, sGnRH-II, and NR1 in the olfactory bulb and telencephalon of male and female adult chum salmon were normalized to the reference gene (β-actin), and the expression levels were compared using the relative Ct (ΔΔCT) method. Data are presented as the means ± SEM (N = 10). Different letters represent significant differences using the Steel-Dwass test (sGnRH-I and sGnRH-II in OB) and the Tukey-Kramer multiple comparison test (sGnRH-I, sGnRH-II in TE, and NR1) (p < 0.05).
