Figure 2: Analysis of organelle-specific proteins in total HepG2 cell lysates and in mitochondrial fractions obtained by differential centrifugation (DC), ultracentrifugation (UC) and magnetic bead-assisted isolation (MACS). | Scientific Reports

Figure 2: Analysis of organelle-specific proteins in total HepG2 cell lysates and in mitochondrial fractions obtained by differential centrifugation (DC), ultracentrifugation (UC) and magnetic bead-assisted isolation (MACS).

From: Purity matters: A workflow for the valid high-resolution lipid profiling of mitochondria from cell culture samples

Figure 2

A representative western blot of 2 mitochondria isolations per method is shown. Mitochondrial membrane ATP synthase 5 (ATP5) was used as a marker for mitochondria. For the detection of potential contaminations, antibodies against Calnexin (Calx), Histone H3 and Giantin were used.

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