Table 1 Summed content of lipid classes quantified by LC-MS lipid profiling of mitochondria purified using different isolation methods (pmol/μg protein).

Lipid (sub-) class	DC		UC		MACS		UC vs. DC	UC vs. MACS	DC vs. MACS
Lipid (sub-) class	Mean	SD	Mean	SD	Mean	SD	p-value	p-value	p-value
CE	0.012	0.001	0.019	0.007	0.033	0.017	n.s.	n.s.	0.0135
CER	2.838	0.404	2.447	0.326	2.703	0.662	n.s.	n.s.	n.s.
CL	8.623	1.779	34.433	10.814	5.217	1.522	<0.0001	<0.0001	n.s.
DAG	0.092	0.007	0.129	0.018	0.283	0.077	n.s.	0.0002	<0.0001
FFA	59.601	10.134	60.664	11.180	87.242	28.114	n.s.	n.s.	n.s.
HexCER	1.013	0.076	0.516	0.113	0.719	0.151	<0.0001	0.0282	0.0033
LPC	1.625	0.459	1.834	0.476	1.709	0.727	n.s.	n.s.	n.s.
LPE	0.728	0.163	0.838	0.251	0.699	0.249	n.s.	n.s.	n.s.
LPI	0.081	0.032	0.064	0.010	0.052	0.021	n.s.	n.s.	n.s.
PC	91.406	5.802	86.358	11.261	89.657	14.345	n.s.	n.s.	n.s.
PE	72.193	17.171	85.393	16.767	60.249	24.064	n.s.	n.s.	n.s.
PE-P	1.313	0.243	0.526	0.136	0.916	0.166	<0.0001	0.0064	0.0078
PG	6.198	1.495	12.973	4.579	3.582	1.639	0.0061	0.0003	n.s.
PI	41.717	7.223	39.157	8.012	44.678	9.599	n.s.	n.s.	n.s.
PS	11.296	1.444	1.200	0.335	8.494	1.630	<0.0001	<0.0001	0.0066
SM	8.185	0.619	3.090	0.662	5.834	1.642	<0.0001	0.0020	0.0092
TAG	17.082	1.565	7.313	0.964	53.924	11.260	n.s.	<0.0001	<0.0001

DC, differential centrifugation; UC, ultracentrifugation; MACS, magnetic bead-assisted isolation; CE, cholesteryl ester; CER, ceramide; CL, cardiolipin; DAG, diacylglycerol; FFA, free fatty acids; HexCER, hexosyl-ceramide; LPC, lyso-phosphatidylcholine; LPE, lyso-phosphatidylethanolamine; LPI, lyso-phosphatidylinositol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PE-P, phosphatidylethanolamine plasmalogen; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin; TAG, triacylglycerol. n.s., not significant. Values are means of at least 5 purifications.

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