Figure 3
From: PNA lectin for purifying mouse acinar cells from the inflamed pancreas
PNA-purified acinar cells contain fewer contaminating mesenchymal and inflammatory cells.
The purity of acinar cells that were FAC sorted using UEA-I or PNA from C57BL/6 mice, or by tomato from Ela-Cre; TOM mice was compared to the unlabeled dissociated pancreatic cells from C57BL/6 mice by examination of mRNA levels of specific cell marker genes, including amylase (for acinar cells), SYN (for endocrine cells), CK19 (for duct cells), CD31 (for endothelial cells), vimentin (for mesenchymal cells) and CD45 (for inflammatory cells). *p < 0.05. NS: non-significant. N = 5.
