Figure 2: Pretreatment of HaCaT cells with HO-1 activators Hemin or CoPP attenuates Th17 cytokine-induced keratinocyte hyperproliferation. | Scientific Reports

Figure 2: Pretreatment of HaCaT cells with HO-1 activators Hemin or CoPP attenuates Th17 cytokine-induced keratinocyte hyperproliferation.

From: Heme oxygenase-1 induction attenuates imiquimod-induced psoriasiform inflammation by negative regulation of Stat3 signaling

Figure 2

(a) HaCaT cells were seeded in 96-cell plates, and stimulated with 10, 25, 50, and 100 ng/mL IL-6 or IL-22 for 24 h before measuring cytokine-induced keratinocyte proliferation using MTS assay, which was based on the cell viability assay. (b) HaCaT cells were seeded in 96-cell plates, and stimulated with 10, 25, 50, and 75 nM Hemin or CoPP for 24 h before measuring cytokine-induced keratinocyte proliferation using MTS assay, which was based on the cell viability assay. (c) HaCaT cells were stimulated with 25 ng/mL IL-6/IL-22 for 24 h in the presence or absence of 25 μM Hemin or CoPP. Total cell numbers were assessed based on the cell viability assay detected using the MTS methods. *represents P < 0.05, which indicates a statistically significant difference. **represents P < 0.005.

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