Figure 3: HO-1 activation in HaCaT cells inhibits Th17 cytokine-induced Stat3 phosphorylation and downstream gene expression. | Scientific Reports

Figure 3: HO-1 activation in HaCaT cells inhibits Th17 cytokine-induced Stat3 phosphorylation and downstream gene expression.

From: Heme oxygenase-1 induction attenuates imiquimod-induced psoriasiform inflammation by negative regulation of Stat3 signaling

Figure 3

(a) HaCaT cells were pretreated with 25 μM Hemin for 24 h before exposure to 25 ng/mL IL-6 or IL-22 for 30 min. Whole-cell extracts were collected, and Stat3 activation (PY-Stat3) was determined. (b) HaCaT cells were pretreated with 25 μM CoPP for 24 h before exposure to 25 ng/mL IL-6 or IL-22 for 30 min. (c) HaCaT cells were treated with 25 μM Hemin for 12 h before exposure to 25 ng/mL IL-6 or IL-22 for 24 h. Whole-cell extracts were collected, and the expression levels of Stat3 downstream genes (Mcl-1, survivin, and Bcl-2) and abnormal keratin (keratin 16 and keratin 17) were determined by Western blot. The blots shown are derived from multiple gels. The gels were run under the same experimental conditions. The membrane was cut based on molecular weight and probed with antibody of interest. Band of interest is indicated with an arrow. All full-length blots are presented in Supplementary Fig. 1.

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