Figure 6: HO-1 inhibited Stat3 activation in normal human keratinocyte through SHP-1 expression. | Scientific Reports

Figure 6: HO-1 inhibited Stat3 activation in normal human keratinocyte through SHP-1 expression.

From: Heme oxygenase-1 induction attenuates imiquimod-induced psoriasiform inflammation by negative regulation of Stat3 signaling

Figure 6

(a) The morphology of cultured primary human keratinocytes was observed under phase–contrast microscope. Magnification: ×100. (b) Immunocytochemistry staining of isolated human keratinocytes with cytokeratin (AE1/AE3) Ab. Magnification: ×400. (c) NHKC were pretreated with 25 μM CoPP for 24 h before exposure to 25 ng/mL IL-22 for 30 min. Whole-cell extracts were collected, and Stat3 activation (PY-Stat3), HO-1 and SHP-1 expression were determined. (d) NHKC were pretreated treated with 25 μ Hemin for 12 h before exposure to 25 ng/mL IL-22 for 24 h. Whole-cell extracts were collected, and the expression levels of Stat3 downstream genes survivin and abnormal keratin (keratin 16 and keratin 17) were determined by Western blot. Blots shown are derived from multiple gels. The gels were run under the same experimental conditions. The membrane was cut based on molecular weight. All full-length blots are presented in Supplementary Fig. 4.

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