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Figure 1

From: Volatile Oil of Acori Graminei Rhizoma-Induced Apoptosis and Autophagy are dependent on p53 Status in Human Glioma Cells

Figure 1

VOA induced differential cell death in human glioma cells and normal cells.

(A) p53 wild-type cells (A172 and U87), p53 mutant cells (U251 and U118) and normal cells (HEB and NIH/3T3) were treated with 0, 25, 50, 75, 100, 150, 175, 200 and 250 μg/ml VOA for 48 or 72 h and cell viability were determined by SRB assay. (B) Colonies in A172 and U251 cells were treated with indicated concentrations of VOA and allowed to grow for 2 weeks before stained with 0.005% crystal violet. Adjacent picture depicts the crystal violet-stained colonies and bar graph indicated the cloning efficiency compared with untreated control. Values represent mean ± SD. *p < 0.05 compared with control.

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