Figure 4
Autophagy was triggered in both A172 and U251 cells by VOA.
(A) A172 and U251 cells were treated with indicated concentrations of VOA for 48 h. Then, expression of autophagy related proteins including LC3II/I, p62, atg 5 and beclin 1 was detected by Western blotting. The blots were a representative of three independent experiments. (B) A172 and U251 cells were transfected with GFP-LC3 for 6 h and then treated with VOA (100 μg/ml for A172 cells and 200 μg/ml for U251 cells) for 48 h. A punctate distribution of LC3 in both cells was observed by fluorescence microscope (400×). The scale of bar is 100 μm. (C) A172 and U251 cells were treated with indicated concentrations of VOA for 48 h, then stained by acridine orange and observed under fluorescence microscope (200×). The scale of bar is 100 μm.
