Table 1 Genomic DNA analysis of integration sites in 13 selected PTS (phototaxis-screening) mutants.

From: Microfluidic high-throughput selection of microalgal strains with superior photosynthetic productivity using competitive phototaxis

Mutant

Location

Protein ID b

Function c

Methods for sequence determination

PTS1

promoter (B) a

516748

Signal recognition particle receptor

TAIL-PCR & PCR-SP d

PTS23

promoter (B)

525919

Similar to homeobox A3 (Mus musculus)

Inverse PCR & PCR-SP

PTS26

17th intron (B)

516786

Ionotropic glutamate receptor

TAIL-PCR & PCR-SP

PTS36

5′-UTR (B)

519251

Putative glycine-rich cell wall structural protein

TAIL-PCR & PCR-SP

PTS37

5′-UTR (B)

519327

Glycoside hydrolase

TAIL-PCR & PCR-SP

PTS42

promoter (B)

516641

Protein binding; PHD-type zinc finger

TAIL-PCR & PCR-SP

PTS59

3rd intron (S)

523869

Diacylglycerol acyltransferase family

TAIL-PCR

PTS61

3′-UTR (B)

513005

Vesicle-mediated transport; t-SNARE family

TAIL-PCR & PCR-SP

PTS64

2nd intron (B)

520695

Biopterin transport-related protein BT1

TAIL-PCR & PCR-SP

PTS66

3′-UTR (B)

518826

Flagellar associated protein

TAIL-PCR & PCR-SP

PTS69

5′-UTR (S)

515661

3-phosphoshikimate 1-carboxyvinyltransferase

TAIL-PCR

PTS118

5′-UTR (B)

513996

Nucleosome assembly; Histone H4

TAIL-PCR & PCR-SP

PTS124

6th exon (B)

512634

GDP mannose transporter

TAIL-PCR & PCR-SP

  1. aB: both flanking sequences were identified, S: single flanking sequence was identified.
  2. bProtein IDs were obtained from JGI Chlamydomonas v4.0 (encoded by gene closest to the insertion site).
  3. cFunction (domain, motif) was described based on the JGI Chlamydomonas v4.0.
  4. dPCR-SP: PCR using specific primers (Supplementary Table S1).