Figure 2
Expression of H. pylori bioV (HP0287) complements growth of an E. coli strain containing a deletion of bioH (ΔbioH).
Transformants of strain STL24 (an E. coli ∆bioH strain) were grown at 37 °C on biotin-free medium with glycerol as carbon source. Growth in the absence of biotin was tested in either the presence or the absence of 0.02% arabinose (Ara) and comparable growth was obtained on the two media (bottom two plates). Glycerol gives basal expression of BioV whereas arabinose gives induced expression (a roughly 50-fold increase). The strains tested were: wild type (MG1655, upper left sectors), STL24 carrying plasmid pBHK565 encoding bioV (lower right sectors), pBHK566 encoding HP0288 (lower left sectors) or the empty pBAD24M vector (Vec) plasmid (upper right sectors). The right hand two plates both lacked arabinose induction whereas the top plate contained biotin as a control.
