Figure 2

Characterization of fc-rPEI-Cdots by (A) H¹NMR and (B) FT-IR. From H¹NMR, disappearance of alkenyl proton peaks at 5.6 and 6.2 ppm (peak a and b) and preservation of peak at 2.8 ppm (peak d) proved successful synthesis of rPEI-Cdots. Appearance of proton resonance peak between 1.8 ppm showed conjugations of folate. From FT-IR, preservation of C-N and N-H stretching signals of PEI-Cdots (ν = 1018 cm⁻¹and 3378 cm⁻¹), amide C = O stretching (ν = 1684 cm⁻¹) signal of BAC and appearance of phenyl absorption peak of folic acid at 1470 cm⁻¹ and 1513 cm⁻¹ in the spectrum of fc-rPEI-Cdots demonstrated successful synthesis of fc-rPEI-Cdots.