Figure 5: Blue light does not promote DNA damage in microglia. | Scientific Reports

Figure 5: Blue light does not promote DNA damage in microglia.

From: Blue Light Modulates Murine Microglial Gene Expression in the Absence of Optogenetic Protein Expression

Figure 5

N9 microglia were exposed to no light (0 mJ/cm2; control, top row) or blue light (450 nm) at 50 mJ/cm2 (middle row) and 100 mJ/cm2 (bottom row) for 1 sec/min for 6 hours, after which time they were stained with anti-γ-H2AX antibodies (left column). Brightfield images of the same field-of-view are shown in the middle column. As a positive control for DNA strand breaks, cells were also exposed to 100 μM etoposide and stained with anti-γ-H2AX antibodies (right column). No dose of light tested induced DNA damage, and blue light did not further increase etoposide-induced DNA damage. Scale bar = 50 μm.

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