Figure 6: Blue light delivered in an optogenetic pattern decreases LPS-stimulated microglial inflammatory gene expression. | Scientific Reports

Figure 6: Blue light delivered in an optogenetic pattern decreases LPS-stimulated microglial inflammatory gene expression.

From: Blue Light Modulates Murine Microglial Gene Expression in the Absence of Optogenetic Protein Expression

Figure 6

The effect of optogenetic light delivery on the basal expression of (a) pro-inflammatory and (b) anti-inflammatory/growth factor genes (n = 6), or LPS-induced expression of (c) pro-inflammatory and (d) anti-inflammatory/growth factor genes (n = 6) is shown. 15 ms pulses of blue light (450 nm) was delivered at 10 Hz, (~25 mW/cm2) for 6 hrs to primary microglia treated with vehicle (a,b) or 1 μg/mL LPS (c,d). Data are graphed as means + 1 SEM of light-induced % change in gene expression relative to the no light controls (dotted line; a,b) or light-induced % change in LPS-stimulated gene expression relative to LPS alone in the absence of light (dotted line; c,d). *P < 0.05, **P < 0.01, ***P < 0.001 vs. respective to no light controls by Holm-Sidak test.

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