Figure 1
From: ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs
Differentiation of bmMSCs into adipocytes under macromolecular crowding (MMC) upregulates UCP1 expression.
(a) Fluorescence images of Nile Red-stained lipid droplets (yellow) and DAPI-stained nuclei (blue) at 10X magnification. Scale bar: 200 μm. (b) qPCR analysis of pan-adipocyte genes: FABP4 = fatty acid binding protein 4, HSL = hormone sensitive lipase, LEP = leptin, GLUT4 = glucose transporter type 4. (c) qPCR analysis of BAT selective genes: UCP1 = uncoupling protein 1, PRDM16 = PRD1-BF1-RIZ1 homologous domain containing 16, CIDEA = cell death-inducing DNA fragmentation factor, alpha subunit-like effector a. (d) Quantification of mitochondrial mass using mitotracker Green normalized to cell number. Data is expressed as mean ± SEM. n.s. =not significant; *p < 0.05; **p < 0.01; ***p < 0.001. N = 3.
