Figure 7
From: ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs
3D microenvironment promotes Col IV cocoon formation and brown adipogenesis in bmMSC-derived adipocytes.
bmMSCs were differentiated for 2 weeks on TCP, bovine collagen I coating and hydrogel. (a) Fluorescence images of Col IV (red), lipid droplets (green) and DAPI-stained nuclei (blue) at 64× magnification. Scale bar: 20 μm. (b) qPCR analysis of: UCP1 = uncoupling protein 1, PGC1α = PPARγ co-activator 1α, DIO2 = deiodinase, iodothyronine, type II; FABP4 = fatty acid binding protein 4 and LEP = leptin. Data is expressed as mean ± SEM. n.s. =not significant; *p < 0.05; **p < 0.01; ***p < 0.001. N = 3.
