Figure 8
From: ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs
Browning of WAT-differentiated bmMSCs with MMC.
bmMSCs were subjected for 6 weeks to either a white or brown protocol in the presence or absence of MMC. A third group of cultures was subject to a white or brown protocol for 3 weeks and then switched to MMC or a brown protocol with MMC. Conversion of white to brown was monitored by UCP1 mRNA expression. (a) UCP1, (b) FABP4 and (c) LEP expression were determined by qPCR. UCP1 = uncoupling protein 1, FABP4 = fatty acid binding protein 4, LEP = leptin. Data is expressed as mean ± SEM. n.s. =not significant; *p < 0.05; **p < 0.01; ***p < 0.001. N = 3.
