Figure 2
From: Three-dimensional Organotypic Culture Models of Human Hepatocellular Carcinoma
(A) EpCAM expression level in Huh1 cells treated with control shRNA or EpCAM shRNA were determined by quantitative RT-PCR (top panel) and western blotting (bottom panel). RT-PCR data are presented as the average of four independent readings. (B) Relative number of Huh1 spheres treated with control shRNA or EpCAM shRNA is shown. Black bars and grey bars indicate relative number of total spheres and acini, respectively, based on the total number of control spheres. The percentage value shown above each grey bar indicates the ratio of acini to total spheres. Data are presented as the average of triplicate experiments. (C) β-catenin luciferase reporter activity in 2D cells (upper left panel) and 3D spheroids (middle left panel), EpCAM and Cyclin D1 expression level in 3D spheroids (upper right panel), total number of spheres (bottom left panel) and the proportion of acini (bottom right panel) treated with DMSO, AV-606 (AV, 3 μM) and fiduxosin (FDS, 10 μM) are shown. Data are presented as the average of at least three independent experiments. (D,E) Relative caspase 3/7 activity of 2D or 3D-cultured Huh1 cells treated with anti-cancer drugs (D, 5-FU; E, doxorubicin). Data are presented as the average of three independent analyses. All error bars shown represent ±SEM. **p < 0.01, ***p < 0.001.
