Table 1 Estimates of critical electric field [kV/cm] magnitude (±ΔEcrit) for electroporation of C. glutamicum, M. smegmatis, and E. coli BL21 exposed to a single exponentially decaying (t = 1.0 ms; τ = 5.0 ms) pulse in 0.01× PBS buffer with 5 μM SYTOX® Green nucleic acid stain.
From: Microfluidic Screening of Electric Fields for Electroporation
Trial | Vapplied [kV] | C. glutamicum | M. smegmatis | E. coli BL21 | |||
|---|---|---|---|---|---|---|---|
E crit | ΔEcrit | E crit | ΔEcrit | E crit | ΔEcrit | ||
1 | 1.0 | – | – | – | – | 4.06 | 0.03# |
2 | 1.0 | – | – | – | – | 4.21 | 0.08 |
3 | 1.0 | – | – | – | – | 3.59 | 0.15 |
4 | 1.0 | – | – | – | – | 3.97 | 0.06 |
5 | 1.5 | – | – | – | – | 2.89 | 0.09 |
6 | 1.5 | – | – | – | – | 2.94 | 0.06 |
7 | 1.5 | – | – | – | – | 3.52 | 0.03 |
8 | 2.0 | 5.53 | 0.21 | 4.59 | 0.03 | 3.52 | 0.10 |
9 | 2.0 | 4.19 | 0.16 | 6.33 | 0.05 | 4.23 | 0.19 |
10 | 2.0 | 4.98 | 0.16 | *6.31 | 0.14 | 3.55 | 0.07 |
11 | 2.5 | 5.47 | 0.15 | 5.44 | 0.11 | – | – |
12 | 2.5 | 5.58 | 0.32 | *4.13 | 0.12 | – | – |
13 | 2.5 | 5.42 | 0.19 | 6.53 | 0.05# | – | – |
Average | – | 5.20 | 0.20 | 5.56 | 0.08 | 3.65 | 0.09 |
