Figure 3
From: Hyporesponsiveness to the anti-inflammatory action of interleukin-10 in type 2 diabetes
RAW264.7 cells and BMDMs (bone marrow-derived macrophages) grown in 15 mM glucose are hyporesponsive to IL10.
RAW264.7 cells (a) and BMDMs (b) were cultured for six hours in 9% FCS in DMEM containing either 5 mM or 15 mM glucose. Cells were then stimulated for two hours with 1 ng/mL LPS in the presence of 0–10 ng/mL of IL10 and supernatants collected for TNF-α production (expressed as % of that induced by LPS alone) by ELISA. Values are mean ± SEM (N = 3). *p < 0.05 & **p < 0.001 vs. 5 mM glucose condition.
