Figure 6
From: Hyporesponsiveness to the anti-inflammatory action of interleukin-10 in type 2 diabetes
P-AMPK levels are not affected by IL10 treatment in RAW264.7 cells.
RAW264.7 cells were cultured for six hours in either 5 mM or 15 mM glucose. Duplicate sets of cells were then stimulated with 1 ng/mL IL10. (a) Cell lysates were separated by SDS-PAGE gel and immunoblotted with antibodies to P-AMPKα (Thr-172) and total AMPKα protein. (b) Band intensities were quantified on a Li-Cor Odyssey scanner and P-AMPKα/total AMPKα protein ratios shown in the bar graph (no effects of time or condition, all P > 0.05) Values are mean ± SEM (N = 3).
