Figure 7
From: Hyporesponsiveness to the anti-inflammatory action of interleukin-10 in type 2 diabetes
AQX-MN100 can bypass IL10 hyporesponsiveness to inhibit inflammation in cells grown in high glucose.
Cells were cultured for six hours in 9% FCS in DMEM containing either 5 mM (Low) or 15 mM (High) glucose. Cells were then stimulated for two hours with 1 ng/mL LPS in the presence of 10 ng/mL IL10 or 50 uM AQX-MN100. TNF-α production was assessed by ELISA. IL10 was less effective in high glucose whereas the anti-inflammatory actions of AQX-MN100 were not influenced. Values are mean ± SEM (N = 3). **p < 0.001 as compared to low glucose.
