Figure 1 | Scientific Reports

Figure 1

From: “Optical communication with brain cells by means of an implanted duplex micro-device with optogenetics and Ca2+ fluoroimaging”

Figure 1

(a) Schematic diagram of a concept for bidirectional, optical neurocommunication. After gene-transfer of the photosensory gene and fluo-indicator gene into the cell, (1) the cell was stimulated by a blue LED and (2) excited by a green LED. (3) The dynamic change in red fluorescence emitted from the cell, which passed through an absorption filter, was imaged using a CMOS sensor. The fluorescence image labeled “cultured cells” shows Neuro2a cells, which were differentiated and matured to form neurites by long-term culture (see Fig. 3 for details) and “animal” is the frozen section of the mouse brain at the visual cortex region stained with DAPI. Gene-transfer in the mouse was accomplished using in utero electroporation (see Fig. 6 for details). (b) This diagram shows the control system of the bidirectional light transmission device. The device is connected to a control board through a transit board and is controlled by a single PC.

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