Figure 3

Requirement study of the 3D culture condition of Neuro2a cells with collagen-gel or matrix-gel, on the dish or on-chip.

(a) Neuro2a cells transfected with green fluorescent protein gene were cultured with seven different media in a 24-well plate and images were taken using an inverted fluorescence microscope. After culture for 5 days, the proliferated cells and the cells that had neurite-like structures were counted; totals are plotted in the graph. (b) The transfected Neuro2a cells were cultured three-dimensionally in three different gels in a glass bottom dish. After culture for 5 days, images were taken using an inverted fluorescence microscope. (c) Neuro2a cells were cultured in ECM gel on the sensor chip. After culture for 5 days, images were taken using a stereo fluorescence microscope. The upper image is magnified the under image.